Study on the Response of Lepista sordida Sucrose Invertase 1 to Sugar Signals
School of Biological Science and Agriculture, Qiannan Normal University for Nationalities, Duyun 558000, China
| Abstract: | Lepista sordida, a rare edible and medicinal mushroom, is currently in short supply in the market due to its declining wild availability and limited artificial cultivation. During cultivation, it has been observed that the mycelial growth rate is slow, making it imperative to explore methods for enhancing mycelial growth. As an essential energy source for edible fungi, the efficient conversion and utilization of carbon substrates significantly influence mycelial growth and fruiting body yield. Sucrose invertase (INV1), a hydrolase belonging to the glycosidase family, catalyzes the hydrolysis of sucrose into equimolar amounts of glucose and fructose. The resulting monosaccharides can be directly absorbed by cells and utilized for energy metabolism or biosynthetic processes. Studying this enzyme will provide a theoretical foundation for investigating the mycelial growth rate of Lepista sordida. In this study, a wild strain of L. sordida was obtained through tissue isolation, and the CDS and amino acid sequences of INV1 were acquired from the Institute of Biological and Medical Engineering, Guangdong Academy of Sciences, laying the groundwork for subsequent research. Using techniques such as reverse transcription quantitative PCR (RT-qPCR), the expression responses of L. sordida under sugar-free, glucose, lactose, and PDA culture conditions were examined. The results indicated that INV1 expression was lowest in the presence of glucose, moderate under PDA and sugar-free conditions, and highest when lactose was supplied as the carbon source. This suggests that sucrose invertase in L. sordida responds to lactose conversion when lactose is the sole carbon sourc. |
| Keywords: | Lepista sordida; Tissue Isolation; ITS Sequence (Internal Transcribed Spacer Sequence); Sucrose Invertas; Carbon Source |
| DOI: | 10.57237/j.jaf.2025.04.003 |
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Received: 25 November 2025
Accepted: 15 December 2025
Published Online: 24 December 2025
Funding
| 1. | 贵州省科技厅青年引导项目 (黔科合基础-[2024]青年054) |
| 2. | 黔南民族师范学院支持高层次人才引进研究专项 (qnsyrc202312) |
| 3. | 黔南民族师范学院大学生创新创业训练计划项目 (qnsycxcy202574) |
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