The Biological Effects of Toxic Dose Combustion Cigarettes and Heated Tobacco Products in BEAS-2B and A549 Cells
1. Shanghai New Tobacco Product Research Institute Co., Ltd., Shanghai 201315, China
2. Technology Center, Shanghai Tobacco Group Co., Ltd., Shanghai 201315, China
| Abstract: | The use of novel tobacco products that do not burn tobacco leaves is becoming increasingly popular worldwide. In terms of respiratory system, smoking damage to the epithelial cells of lung is in urgent need of attention and research. In this study, we produced the combustion cigarettes (CC) condensate from conventional cigarettes and the heated tobacco products (HTP) condensate from heat-not-burn (HNB) tobacco, and determine the nicotine and other ingredients content respectively to assessment the CC and HTP cytotoxicity after acute exposure to cigarette smoke. We further select a toxicological dose for comparison of the difference between the CC and HTP, which the impacts on BEAS-2B and A549 cells at a 70% survival rate was evaluated. Then, biological effects of CC and HTP on BEAS-2B and A549 cells, which including cell viability, apoptosis and cell cycle were investigated. Results showed that the BEAS-2B and A549 cells survival rate was decreased with increasing dose of CC and HTP smoke trap for 24 h. Both CC and HTP could suppress the proliferation of BEAS-2B cells, though the degree of inhibition of cell proliferation in CC group was greater than in HTP group. The CC and HTP traps could promote apoptosis in BEAS-2B and A549 cells. The apoptosis rate of HTP group was less than that of CC group. In addition, CC and HTP traps could partly restrain cell cycle progression in G0/G1 phase. These results demonstrate that CC and HTP can cause cytotoxicity, apoptosis and affect cell cycle progression, which thus may be useful to elucidate the underlying biological effects of novel tobacco products and provide new ideas for the preventing and treating diseases. |
| Keywords: | Combustion Cigarettes (CC); Heated Tobacco Products (HTP); Toxicity; Lung Cells |
| DOI: | 10.57237/j.life.2024.02.001 |
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Received: 11 March 2024
Accepted: 28 March 2024
Published Online: 13 April 2024
Funding
| 1. | Shanghai New Tobacco Product Research Institute Technology Project Foundation (K2023-009Z) |
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