Uncertainty Evaluation and Analysis of Enterobacteriaceae Count in Proficiency Testing
Hainan Institute for Food Control (Hainan Experimental Animal Center), Key Laboratory of Tropical Fruits and Vegetables Quality and Safety for State Market Regulation, Haikou 570314, China
| Abstract: | Objective: To analyze and evaluate the uncertainty the results of the proficiency testing (ACAS-PT 1083 (2021)) of enterobacteriaceae count. Methods: According to the operational instructions of capability verification and GB 4789.41-2016 National food safety standard-Food microbiology test- Enterobacteriaceae count, the enterobacteriaceae of the capability verification sample were tested quantitatively. The suspected colonies were identified by VITEK2. The uncertainty of test results was assessed in accordance with JJF 1059.1-2012 and GB/T 27418-2017 Evaluation and expression of measurement uncertainty. Results: The test result of sample 21-Q997 was 4.2 ×103 CFU/mL. The test results were satisfactory as the Z values of the proficiency test was -0.8 (|Z| was less than 2.0). The extended uncertainty of enterobacteriaceae test results was 0.0288 at 95% confidence interval. The VITEK 2 identification system identified suspicious colonies as E. coli with 99% confidence. Conclusion: The evaluation results demonstrated that the laboratory had the ability to detect the count of enterobacteriaceae in food and the accuracy of VITEK2's auxiliary identification of suspicious colonies has been verified. The evaluation method established in this study can evaluate the uncertainty of the quantitative detection of enterobacteriaceae count with ability verification, and is suitable for the evaluation of the relevant uncertainty in laboratories with similar testing conditions. |
| Keywords: | Proficiency Testing; Enterobacteriaceae; Uncertainty Evaluation |
| DOI: | 10.57237/j.wjfse.2023.02.001 |
View:
Downloads:
Received: 26 April 2023
Accepted: 25 May 2023
Published Online: 15 June 2023
Funding
| 1. | 海南省自然科学基金项目资助 (321QN0971) |
| 2. | 国家市场监管重点实验室 (热带果蔬质量与安全) 自主课题 (ZZ-2022010) |
References
| [1] | 张西萌, 付溥博, 魏咏新, 等. *3M PetrifilmTM肠杆菌科测试⽚的评价 [J]. 食品安全质量检测学报, 2021, 12 (04): 1612-1619. |
| [2] | 刘瑛, 俞静, 陈峰, 等. MicroflexTM MALDI-TOF MS 和 Vitek 2 Compact 全自动微生物分析系统对肠杆菌科细菌鉴定能力的比较 [J]. 检验医学, 2015, (2): 122–127. |
| [3] | 马群飞. GB 4789.28—2013《食品安全国家标准 食品微生物学检验 培养基和试剂的质量要求》应用现状 [J]. 中国卫生标准管理, 2019, 10 (16): 1–3. |
| [4] | 房海, 陈翠珍, 张晓君. 肠杆菌科病原细菌 [M]. 北京:中国农业科学技术出版社, 2011. |
| [5] | 李点, 董明盛, 张秋勤. 肠杆菌科细菌群体感应系统的研究进展 [J]. 生物加工过程, 2020, 18 (02): 193-199. |
| [6] | 揭琴丰, 李露敏, 邱伟华, 等. 江西省婴儿配方羊乳粉生产加工过程肠杆菌科和克罗诺杆菌属污染情况调查 [J]. 当代医学, 2019, 25 (25): 24-27. |
| [7] | 孙建云, 胡晓宁, 兰光, 等. 婴儿配方粉及生产加工环节微生物污染情况调查 [J]. 中国食品卫生杂志. 2017, 29 (04): 474-477. |
| [8] | 段锦淼, 高庆超, 李亚辉等. 我国与国际食品法典委员会和欧盟婴幼儿食品安全标准中风险因子限量差异分析与思考 [J]. 食品科学. 2022, 43 (09): 300-309. |
| [9] | 卢行安, 顾其芳, 袁宝君, 等. AOAC PetrifilmTM菌落总数测试片法与食品中菌落总数测定国标方法的比较研究 [J]. 中国食品学报. 2011, 11 (03): 164-167. |
| [10] | GB 23790-2010食品安全国家标准粉状婴幼儿配方食品良好生产规范 [S]. |
| [11] | 韦云, 陈佩虹, 周露. 矿泉水中铜绿假单胞菌的测定能力验证结果分析 [J]. 食品安全质量检测学报, 2020, 11 (16): 5398-5402. |
| [12] | 刘笑笑, 蔡玉红, 于丽, 等. 多管发酵法测定生活饮用水中总大肠菌群不确定度的评定 [J]. 中国食品添加剂, 2019, (5): 123-127. |
| [13] | 虹涛, 李青. 不确定度评定在微生物检验中的应用 [J]. 中国卫生检验杂志, 2020, 30 (16): 2046-2048. |
| [14] | ACAS-PT 1083食品中大肠埃希氏菌、粪大肠菌群、肠杆菌科计数的检验能力验证参试指导书 [Z]. |
| [15] | GB 4789.41-2016《食品安全国家标准食品微生物学检验 肠杆菌科检验》 [S]. |
| [16] | 秦文, 王丽媛, 王晶波, 等. 酸奶中乳酸菌计数不确定度的评定 [J]. 中国卫生检验杂志, 2018, 28 (15): 1844-1846. |
| [17] | 王爱雯, 吴学贵, 周梅秀, 等. 罗非鱼中菌落总数测量结果的不确定度评定 [J]. 食品安全质量检测学报, 2022, 11 (5): 1492-1495. |
| [18] | 郭丽艳. 能力验证样品中霉菌和酵母检测结果的不确定度评定 [J]. 食品安全质量检测学报, 2019, 10 (18): 6088-6092. |
| [19] | JJG 196-2006 常用玻璃量器检定规程 [S]. |
| [20] | 彭祺, 高海峰, 竹惠燕, 等. 三重四级杆串联质谱法测定糯米 (黄酒原料) 中多菌灵残留量不确定度分析 [J]. 酿酒科技, 2014 (01): 93-96. |
| [21] | JJF 1059.1-2012 测量不确定度的评定与表示 [S]. |
| [22] | 邓晓鸿, 强敏, 朱新生, 等. 婴幼儿配方食品中阪崎肠杆菌的计数结果不确定度评定 [J]. 食品安全质量检测学报, 2020, 11 (20): 7476-7481. |
| [23] | 张炜煜, 王岙, 杨修军, 等. 生活饮用水中总大肠菌群MPN法的不确定度评定 [J]. 中国卫生检验杂志, 2019, 29 (21): 2600-2603. |
| [24] | 郑瑞. 肠杆菌科细菌对碳青霉烯类药物耐药特性及环境影响因素研究. [D] 昆明: 昆明理工大学, 2017. |
| [25] | 岳苑, 贾冰凝, 马桂娟. 食品中单核细胞增生李斯特氏菌的测量不确定度评定 [J]. 生物加工过程, 2018, 16 (2): 87-92. |
| [26] | 武利平, 张晓, 丁培, 等. 能力验证菌落总数结果测量不确定度评定分析 [J]. 中国卫生检验杂志, 2017, 27 (24): 3545-3547. |
| [27] | 游元丁, 赵阳, 林雪丹, 等. 食品中金黄色葡萄球菌测量不确定度评定 [J]. 食品安全质量检测学报, 2021, 12 (6): 2468-2473. |
| [28] | 赵丽, 胡宝翠, 王琳琳, 等. 糕点中菌落总数测量结果的不确定度的评定 [J]. 食品安全质量检测学报, 2022, 11 (14): 4837-4840. |
Join the Editorial Board
We invite active, qualified and high profile scientists and researchers to join as Editorial Board Members.
Join UsBecome a Reviewer
Scholars with a strong interest in reviewing are invited to join the reviewer panel to ensure the quality of the research to be published.
Join Us