Changes of Proliferation and Secretion of Human Gingival Fibroblasts in Different Glucose Concentrations: An in vitro Study
1. Department of Stomatology, Shanxi Bethune Hospital, Shanxi Academy of Medical Sciences, Third Hospital of Shanxi Medical University, Taiyuan 030053, China
2. Department of Endocrinology, Shanxi Bethune Hospital, Shanxi Academy of Medical Sciences, The Third Hospital of Shanxi Medical University, Taiyuan 030053, China
| Abstract: | Background: To investigate the changes of the proliferation of human gingival fibroblasts (HGFs) and the secretions of Type I collagen (COL1), Type III collagen (COL3) and fibronectin (FN) in different glucose concentrations. Methods: HGFs were cultured in the cell culture medium with different glucose concentrations (5.5 mmol/L (group A), 8.8 mmol/L (group B), 10 mmol/L (group C), and 15 mmol/L (group D)) for 7 days. The proliferation of HGFs was measured using an MTT method, and COL1, COL3, and FN were tested with ELISA. The results and their correlations were statistically analyzed. Results: As the glucose concentrations increased, the quantities of HGFs proliferation and various proteins secreted decreased. HGFs proliferation in groups C and D was significantly less than that in groups A and B (P<0.05). The proliferation in group D were significantly less than that in group C (P<0.05). The amount of COL1, COL3, and FN secreted by HGFs in groups C and D were significantly less than those in groups A and B (P<0.05). The secretions in group D were significantly less than that in group C (P<0.05). The positive correlations were shown between HGFs proliferation quantities and secretions of COL1 (r=0.686), COL3 (r=0.693) and FN (r=0.474) (P<0.05). Conclusions: Proliferation of HGFs was inhibited with increasing glucose concentration, and the secretion function of HGFs was reflected in the variation of HGFs proliferation. |
| Keywords: | Gingival Fibroblast; High Glucose; Cell Proliferation; Collagen; Fibronectin |
| DOI: | 10.57237/j.cmf.2024.01.002 |
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收稿日期: 2022-08-15
接受日期: 2022-09-30
在线出版日期: 2024-01-20
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