MIL-101(Fe)对伊立替康的负载：实验和理论研究
天津中医药大学, 中药学院, 天津 300193
| 摘 要： | 本文以伊立替康（Irinotecan，ICA）为模型药物，金属-有机骨架材料MIL-101(Fe)为药物载体，采用浸渍吸附法用MIL-100(Fe)对ICA进行负载，制备ICA@MIL-100(Fe)载药复合物。建立高效液相色谱法（HPLC）测定ICA的含量，将该方法用于ICA@MIL-100(Fe)中药物含量的测定，建立的方法学经过线性关系、精密度、重复性、稳定性和加样回收率考察，线性关系良好，各项考察RSD均小于2%。通过分子模拟进一步考察药物负载于MIL-100(Fe)中的结合位点及其稳定性。负载实验结果表明，ICA浓度1mg/mL，药物与吸附剂质量比mICA:mMIL-101(Fe)=1:1，吸附时间为24 h，ICA在MIL-101(Fe)的载药量为（8.88±0.03）%。分子模拟实验显示当ICA进入到MIL-101(Fe)的四面体结构时，药物与载体之间的结合呈最稳定的优势构象，佐证MIL-100(Fe)是一种适于ICA负载的新型药物载体。 |
| 关 键 词： | 伊立替康; MIL-101(Fe); 药物负载; 分子对接 |
| DOI： | 10.57237/j.cse.2023.04.002 |
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收稿日期: 2023-08-31
接受日期: 2023-10-27
在线出版日期: 2023-10-28
MIL-101(Fe) as a Drug Carrier of Loading Irinotecan: Experimental and Theoretical Studies
College of Chinese Materia Medica, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
| Abstract: | In this study, Irinotecan (ICA) was used as a model drug, and MIL-101(Fe) was used as a drug carrier to load ICA, ICA@MIL-100(Fe) complex was prepared by impregnating adsorption method. A high performance liquid chromatography (HPLC) method was established to determine the content of ICA from ICA@MIL-100(Fe) complex. The established method had good linear relationship, precision, repeatability, stability and sample recovery, and the RSD was less than 2%. Molecular simulation was used to further investigate the site and stability of interaction. Experiment results showed that when the concentration of ICA was 1mg/mL, the mass ratio of drug to carrier was 1:1, the adsorption time was 24 h, the drug loading capacity of ICA by MIL-100(Fe) was (8.88±0.03) %. Molecular simulation experiments showed that when ICA was incorporated into the tetrahedron of MIL-101(Fe), the binding between ICA and MIL-100(Fe) revealed the most stable dominant conformation, which supported the feasibility of MIL-101(Fe) used as a new drug carrier loading ICA. |
| Keywords: | Irinotecan; MIL-101(Fe); Drug Loading; Molecular Docking |
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