HPLC测定PCL-g-PEI载药胶束中葫芦素B的含量
天津中医药大学, 中药学院, 天津 300193
| 摘 要： | 目的：建立PCL-g-PEI聚合物载药胶束中葫芦素B（Cuc B）的含量测定方法。方法：采用溶剂挥发法等制备Cuc B载药聚合物胶束，采用HPLC测定载药胶束中Cuc B的含量。检测色谱条件：采用Agilent C18色谱柱（150 mm×4.6 mm，5 μm）；流动相：乙腈/水/冰醋酸=45：55：0.1（体积比）；流速：1.0 mL/min；检测波长：228 nm；柱温：25°C；进样量：20 μL。结果：Cuc B在1×10-6 ~ 0.1 mg/mL浓度范围内线性关系良好（r=0.9999）；空白PCI-g-PEI载体无干扰；该方法的精密度、稳定性、重复性以及回收率均符合要求。采用溶剂挥发法制备Cuc B载药胶束的包封率和载药量最高。结论：本文建立的Cuc B测定方法专属性强，有较好的精密度和重现性，且加样回收率符合要求；可定量检载药PCL-g-PEI胶束中Cuc B的含量，且可为中药Cuc B相关制剂的含量测定提供参考。 |
| 关 键 词： | HPLC; 聚合物胶束; 葫芦素B; PCI-g-PEI共聚物 |
| DOI： | 10.57237/j.cse.2024.01.004 |
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收稿日期: 2024-02-05
接受日期: 2024-03-15
在线出版日期: 2024-03-18
Determination of Cucurbitin B in PCL-g-PEI Micelle by HPLC
College of Chinese Materia Medica, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
| Abstract: | Objective: To establish a method for the determination of Cucurbitin B in drug-loaded PCL-g-PEI polymer micelles. Methods: Cucurbitin B loaded micelles were prepared by solvent volatilization. HPLC method was used to determine the content of Cucurbitin B in PCL-g-PEI micelles. The separation was performed on Agilent C18 column (150 mm×4.6 mm, 5 μm) with mobile phase consisted of acetonitrile/water/glacial acetic acid=45: 55: 0.1 (V/V/V) at the flow rate of 1.0 mL/min. The detection wavelength was set at 228 nm, and the column temperature was 25°C. Sample volume was 20 μL. Result: The good linearity with Cucurbitin B concentration range was achieved in the rang of 1×10-6~0.1 mg/mL (r=0.9999). Blank PCI-g-PEI micelle carrier had no interference. The precision, stability, repeatability and recovery of the method meet the requirements. Solvent volatilization method was employed to prepare Cucurbitin B drug-loaded micelle, which has the highest drug loading capacity among them. Conclusion: The method for the determination of Cucurbitin B established in this paper has strong specificity, good precision and reproducibility, and the recovery rate meets the requirements. The content of Cucurbitin B in drug-loaded polymer micelles can be quantitatively determined, which provides reference for the content determination of Cucurbitin B related preparations of Traditional Chinese medicine. |
| Keywords: | HPLC; Polymeric Micelle; Cucurbitin B; PCL-g-PEI Polymer |
基金
| 1. | 国家自然科学基金 (81202921) |
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