沙针种子GA3+KT化学激活与物理破皮萌发效应研究
1. 西南林业大学, 林学院, 云南昆明 650224
2. 中国林业科学研究院, 高原林业研究所, 云南昆明 650233
3. 西南林业大学, 生物与食品工程学院, 云南昆明 650224
4. 红河学院, 生物科学与农学学院, 云南蒙自 661199
| 摘 要： | 针对沙针（Osyris lanceolata）种子萌发特性复杂导致的培育成本高、资源可持续利用受限等问题凸显，本研究旨在系统解析其萌发特性，建立分级萌发策略，降低其人工培育的产业化成本，为规模化繁育与资源保护提供解决方案，推动相关特色产业链的开发。以自然结实种子为材料，通过剥种皮、热激、剥除果皮阴干等机械处理与GA3+KT组合浸种，浓度梯度500-1500 mg/L等化学浸种处理方法，结合萌发率（GP）、萌发指数（GI）、同步性（GV）等萌发指数，以及萌发差异性（Anova、Duncan's）、效应量（η2、Cohen's f）评估，筛选最优萌发方案。化学浸种最优处理方案为1500 mg/L GA3+20 mg/L KT浸种24 h（GK9），萌发率达79.77%；物理最优处理方案为剥除种皮，萌发率67.60%（p<0.001）。为提升沙针种子萌发效果，需采用场景适配的分级策略——精细化育苗采用剥除种皮以规避化学污染，规模化生产首选GK9，逆境栽培探索热激+低浓度GA3协同增效。 |
| 关 键 词： | 沙针; 种子萌发; 物理处理; 化学处理 |
| DOI： | 10.57237/j.jaf.2025.04.002 |
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收稿日期: 2025-10-30
接受日期: 2025-12-11
在线出版日期: 2025-12-24
Seed Germination of Osyris lanceolata: Chemical Induction and Scalable Strategies
1. College of Forestry, Southwest Forestry University, Kunming 650224, China
2. Institute of Highland Forest Science, Chinese Academy of Forestry, Kunming 650233, China
3. College of Biology and Food Engineering, Southwest Forestry University, Kunming 650224, China
| Abstract: | As the problems of high cultivation costs and restricted sustainable resource utilization, resulting from the complex germination characteristics of African sandalwood (Osyris lanceolata), have become prominent, this research is designed to systematically analyze these characteristics and develop a graded germination strategy. This initiative seeks to lower industrial production costs, offer solutions for mass cultivation and conservation, and facilitate the growth of associated specialty industries. Using naturally matured seeds, mechanical treatments (seed-coat removal, heat shock, pericarp removal followed by shade-drying) and chemical soaking with GA3+ kinetin (KT) at 500-1500 mg/L were evaluated. Germination percentage (GP), germination index (GI), and synchrony (GV) served as metrics, while ANOVA, Duncan's test, and effect sizes (η2, Cohen's f) were employed to identify the optimal dormancy-breaking protocol. The optimal chemical treatment was soaking seeds in 1500 mg/L GA3 + 20 mg/L KT for 24 h (GK9), achieving a germination rate of 79.77%; the optimal mechanical treatment was seed coat removal, resulting in a germination rate of 67.60% (p<0.001). To enhance the germination performance of O. lanceolata seeds, a scenario-specific, graded strategy is recommended. For precision nursery cultivation, the M1 method is adopted to avoid chemical contamination. Large-scale production should prioritize the GK9 protocol. For cultivation under stressful conditions, the synergistic effect of heat shock combined with a low concentration of GA3 is recommended for further exploration. |
| Keywords: | Osyris lanceolata; Seed Germination; Physical Treatment; Chemical Treatment |
参考文献
| [1] | 曾凡江, 张文军, 刘国军, 等. 中国典型沙漠区主要优势植被的稳定修复途径与可持续经营技术 [J]. 中国科学院院刊, 2020, 35(06): 709-716. https://doi.org/10.16418/j.issn.1000-3045.20200420001 |
| [2] | 陈红刚, 赵文龙, 晋玲, 等. 红花绿绒蒿种子休眠解除的生理生化响应 [J]. 草地学报, 2023, 31(12): 3697-3705. https://doi.org/10.11733/j.issn.1007-0435.2023.12.014 |
| [3] | 陈月红, 汤晓辛, 石慰, 等. 喀斯特针阔混交林优势物种生态位与种间联结——以贵阳市为例 [J]. 干旱区资源与环境, 2024, 38(11): 159-167. https://doi.org/10.13448/j.cnki.jalre.2024.234 |
| [4] | 崔子佳.林木种子休眠现象及催芽技术研究 [J]. 林业勘查设计, 2023, 52(4): 84-88. https://doi.org/10.3969/j.issn.1673-4505.2023.04.022 |
| [5] | 高耀辉, 马明, 魏光普, 等. 楔叶菊种子及幼苗对镧、铈耐受性及富集特性研究 [J]. 干旱区资源与环境, 2022, 36(04): 155-162. https://doi.org/10.13448/j.cnki.jalre.2022.105 |
| [6] | Sajeev N, Koornneef M, Bentsink L. A commitment for life: decades of unraveling the molecular mechanisms behind seed dormancy and germination [J]. The Plant Cell, 2024, 36(5): 1358-1376. https://doi.org/10.1093/plcell/koad328 |
| [7] | Meng T, Zhang Z, Han S, et al. Study on the distribution characteristics of Osyris L. Resources based on specimens [J]. Life Science and Technology, 2024, 3(1): 11-12. https://doi.org/10.57237/j.life.2024.01.002 |
| [8] | 黄振英, 叶学华, 崔清国, 等. 长期生态学研究为沙地生态恢复和适应性管理提供理论与技术支撑 [J]. 中国科学院院刊, 2022, 37(07): 1006-1013. https://doi.org/10.16418/j.issn.1000-3045.20220629002 |
| [9] | 井瑾,王方贞,张瑜,等.干旱胁迫下火棘种子萌发过程及其抗氧化酶活性变化[J].干旱区资源与环境, 2017, 31(09): 135-139. https://doi.org/10.13448/j.cnki.jalre.2017.293 |
| [10] | 久西加, 陈红刚, 王惠珍, 等. 桃儿七种子休眠解除过程中细胞壁代谢及种皮超微结构的变化 [J]. 西北植物学报, 2023, 43(10): 1704-1715. https://doi.org/10.7606/j.issn.1000-4025.2023.10.1704 |
| [11] | 刘梅清, 徐镱文, 刘媛媛, 等. 药用植物种子休眠及萌发特性研究进展 [J]. 中兽医医药杂志, 2023, 42(04): 47-51. https://doi.org/10.13823/j.cnki.jtcvm.2023.075 |
| [12] | 麦尔耶姆古丽•阿布都瓦力, 阿马努拉•依明尼亚孜, 古丽尼哥•塔依尔, 等. 紫苞鸢尾种子萌发特性及其生态适应性[J]. 干旱区资源与环境, 2018, 32(08): 172-177. https://doi.org/10.13448/j.cnki.jalre.2018.253 |
| [13] | 董雪, 辛智鸣, 张冉浩, 等. 沙冬青种子形态和比叶面积沿降水梯度的变化特征 [J]. 干旱区资源与环境, 2019, 33(12): 172-178. https://doi.org/10.13448/j.cnki.jalre.2019.363 |
| [14] | 全昌乾, 汤丹峰, 姜建萍, 等. 基于转录组测序分析两面针种子休眠解除的分子机制 [J]. 中国中药杂志, 2025, 50(01): 102-110. https://doi.org/10.19540/j.cnki.cjcmm.20241014.103 |
| [15] | 尚铁军, 王姝, 张梦醒, 等. 山桐子种子休眠解除及萌发过程中解剖结构分析 [J]. 种子, 2025, 44(01): 140-146. https://doi.org/10.16590/j.cnki.1001-4705.2025.01.140 |
| [16] | Kim S, Ko C H, Kwon H C. Effect of temperature and covering structures in seed dormancy and germination traits of Manchurian striped maple (Acer tegmentosum Maxim.) native to Northeast Asia [J]. Plants, 2025, 14(5): 767. https://doi.org/10.3390/plants14050767 |
| [17] | 宋松泉, 唐翠芳, 雷华平, 等. 种子休眠与萌发调控的研究进展 [J]. 作物学报, 2024, 50(1): 1-15. https://doi.org/10.3724/SP.J.1006.2024.34012 |
| [18] | Nelson D C. The mechanism of host-induced germination in root parasitic plants [J]. Plant Physiology, 2021, 185(4): 1353-1373. https://doi.org/10.1093/plphys/kiab043 |
| [19] | Korell L, Sandner T M, Matthies D, et al. Effects of drought and N level on the interactions of the root hemiparasite Rhinanthus alectorolophus with a combination of three host species [J]. Plant Biology, 2020, 22: 84-92. https://doi.org/10.1111/plb.12977 |
| [20] | 王艳梅, 张小雪, 朱秀征, 等. 林木种子休眠与萌发机制研究进展 [J]. 林业科学, 2021, 57(10): 128-144. https://doi.org/10.11707/j.1001-7488.20211013 |
| [21] | Sharma A, Verma K, Kumar A, Rani S, Chauhan K, Battan B, Kumar R. Delineating the role of host plants in regulating the water and salinity stress induced changes in sandalwood roots [J]. 3 Biotech, 2024, 14(5): 133-133. https://doi.org/10.1007/s13205-024-03979-8 |
| [22] | 吴羚靖. 帝国的知识生产: 20世纪初全球檀香贸易与檀香植物属名之争 [J]. 世界历史, 2022(2): 16-28. https://doi.org/16-28I0002,I0003 |
| [23] | 吴玉, 周亚晶, 胡惠, 等. 种子休眠与解除的分子机制研究进展 [J]. 种子, 2021, 40(05): 63-70. https://doi.org/10.16590/j.cnki.1001-4705.2021.05.063 |
| [24] | Zagorchev L, Stöggl W, Teofanova D, et al. Plant parasites under pressure: Effects of abiotic stress on the interactions between parasitic plants and their hosts [J]. International Journal of Molecular Sciences, 2021, 22(14): 7418. https://doi.org//10.3390/ijms22147418 |
| [25] | 徐宁伟, 路斌, 陈强, 等. 燕山地区叶底珠萌发期耐盐性综合评价 [J]. 干旱区资源与环境, 2023, 37(04): 178-185. https://doi.org/10.13448/j.cnki.jalre.2023.101 |
| [26] | Watson D M, McLellan R C, Fontúrbel F E. Functional roles of parasitic plants in a warming world [J]. Annual Review of Ecology, Evolution, and Systematics, 2022, 53(1): 25-45. https://doi.org/10.1146/annurev-ecolsys-102320-115331 |
| [27] | 杨琦琦, 蒋志荣, 黄海霞, 等. NaCl和Na2SO4胁迫对裸果木种子萌发的影响 [J]. 干旱区资源与环境, 2019, 33(03): 171-176. https://doi.org/10.13448/j.cnki.jalre.2019.092 |
| [28] | Madelaire C B, Franceschini L, Morais D H, et al. Helminth Parasites of Three Anuran Species during Reproduction and Drought in the Brazilian Semiarid Caatinga Region [J]. The Journal of parasitology, 2020, 106(3): 334-340. https://doi.org/10.1645/16-130 |
| [29] | 杨永义, 倪强, 马瑞, 等. 混合盐 (NaCl+NaHCO3) 和NaCl对黑果枸杞种子萌发和幼苗生长的影响 [J]. 干旱区资源与环境, 2019, 33(07): 168-173. https://doi.org/10.13448/j.cnki.jalre.2019.215 |
| [30] | 张爱英, 熊高明, 樊大勇, 等. 三峡水库蓄水对长江干流河岸植物组成的影响 [J]. 长江流域资源与环境, 2018, 27(1): 12. https://doi.org/CNKI:SUN:CJLY.0.2018-01-017 |
| [31] | 赵丁洁, 纪永福, 张莹花, 等. 沙拐枣幼苗生理特性对风速变化的响应 [J]. 干旱区资源与环境, 2019(9): 6. https://doi.org/CNKI:SUN:GHZH.0.2019-09-028 |
| [32] | 赵娜, 贺梦璇, 李洪远. 混合基质添加对土壤种子库萌发特征的影响 [J]. 环境科学研究, 2016, 29(12): 8. https://doi.org/10.13198/j.issn.1001-6929.2016.12.09 |
| [33] | Baskin C C, Baskin J M. Seed dormancy and germination in Myrtaceae: a palaeohistory, tribe, life cycle and geographical distribution perspective [J]. Seed Science Research, 2025: 1-31. https://doi.org/10.1017/S0960258525000066 |
