花脸香蘑蔗糖转化酶INV1响应糖信号的研究
黔南民族师范学院, 生物科学与农学院, 贵州都匀 558000
| 摘 要： | 花脸香蘑（Lepista sordida）作为一种珍稀的食药用菌，随着野生数量越来越少，目前只有少量的人工栽培，市场上供不应求；在对其栽培的过程中发现菌丝体生长缓慢，探究提高菌丝体生长速率势在必行。碳源作为食用菌必不可少的能量来源，对碳源的高效转化利用影响食用菌菌丝体生长与子实体产量。蔗糖转化酶（Sucrose Invertase, INV1）是一种水解酶，属于糖苷酶家族，能够催化蔗糖水解为等量的葡萄糖和果糖，生成的单糖可直接被细胞吸收并用于能量代谢或生物合成。对它的研究将为探究花脸香蘑菌丝体生长速率提供理论参考。本研究通过组织分离法获得了一株野生的花脸香蘑菌株菌丝体，并从广东省科学院生物与医学工程研究所获得花脸香蘑中INV1的CDS序列与氨基酸序列，为后面的研究奠定基础。通过实时荧光定量 PCR（Reverse Transcription Quantitative PCR, RT-qPCR）等技术手段，对花脸香蘑在无糖、葡萄糖与乳糖以及PDA培养条件下的响应情况做了检测。结果表明，在葡萄糖存在的情况下，其表达量最低，在PDA与无糖时表达量居中，而在乳糖的情况下，蔗糖转化酶表达量最高。这说明花脸香蘑中蔗糖转化酶在碳源只有乳糖存在的情况下，会响应乳糖的转化。 |
| 关 键 词： | 花脸香蘑; 组织分离; ITS序列; 蔗糖转化酶; 碳源 |
| DOI： | 10.57237/j.jaf.2025.04.003 |
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收稿日期: 2025-11-25
接受日期: 2025-12-15
在线出版日期: 2025-12-24
Study on the Response of Lepista sordida Sucrose Invertase 1 to Sugar Signals
School of Biological Science and Agriculture, Qiannan Normal University for Nationalities, Duyun 558000, China
| Abstract: | Lepista sordida, a rare edible and medicinal mushroom, is currently in short supply in the market due to its declining wild availability and limited artificial cultivation. During cultivation, it has been observed that the mycelial growth rate is slow, making it imperative to explore methods for enhancing mycelial growth. As an essential energy source for edible fungi, the efficient conversion and utilization of carbon substrates significantly influence mycelial growth and fruiting body yield. Sucrose invertase (INV1), a hydrolase belonging to the glycosidase family, catalyzes the hydrolysis of sucrose into equimolar amounts of glucose and fructose. The resulting monosaccharides can be directly absorbed by cells and utilized for energy metabolism or biosynthetic processes. Studying this enzyme will provide a theoretical foundation for investigating the mycelial growth rate of Lepista sordida. In this study, a wild strain of L. sordida was obtained through tissue isolation, and the CDS and amino acid sequences of INV1 were acquired from the Institute of Biological and Medical Engineering, Guangdong Academy of Sciences, laying the groundwork for subsequent research. Using techniques such as reverse transcription quantitative PCR (RT-qPCR), the expression responses of L. sordida under sugar-free, glucose, lactose, and PDA culture conditions were examined. The results indicated that INV1 expression was lowest in the presence of glucose, moderate under PDA and sugar-free conditions, and highest when lactose was supplied as the carbon source. This suggests that sucrose invertase in L. sordida responds to lactose conversion when lactose is the sole carbon sourc. |
| Keywords: | Lepista sordida; Tissue Isolation; ITS Sequence (Internal Transcribed Spacer Sequence); Sucrose Invertas; Carbon Source |
基金
| 1. | 贵州省科技厅青年引导项目 (黔科合基础-[2024]青年054) |
| 2. | 黔南民族师范学院支持高层次人才引进研究专项 (qnsyrc202312) |
| 3. | 黔南民族师范学院大学生创新创业训练计划项目 (qnsycxcy202574) |
参考文献
| [1] | 隋业璇, 徐伟良, 李春冬, 等. 一株草原野生花脸香蘑菌种培养基的筛选研究 [J]. 农业与技术, 2023, 43(03): 14-17. |
| [2] | 戴玉成, 周丽伟, 杨祝良, 等.中国食用菌名录 [J]. 菌物学报, 2010, 29(01): 1-21. |
| [3] | 胡先运, 李香莉, 张勇民, 等.花脸香蘑菌丝体氨基酸分析 [J]. 氨基酸和生物资源, 2007, (01): 51-52. |
| [4] | 胡先运, 江家志, 罗心毅.花脸香蘑子实体蛋白质营养评价 [J]. 氨基酸和生物资源, 2011, 33(04): 55-57. |
| [5] | 张秀伟, 鲍菊, 蔡甫格, 等.4个贵州野生花脸香蘑菌株的菌丝体生物学特性研究 [J]. 食药用菌, 2023, 31(03): 182-186. |
| [6] | 张京良, 李蓉, 孙炳竹, 等. 花脸香蘑功能饮料的研制 [J]. 食用菌, 2010, 32(02): 61-62. |
| [7] | 郭昱秀, 王军, 栾倩倩, 等. 祁连山地区野生花脸香蘑的鉴定及培养条件研究 [J/OL]. 天津农林科技, 1-8 (2025-11-24). |
| [8] | 万群, 熊维全, 张金容, 等. 1株野生花脸香蘑菌株的鉴定及其培养基筛选 [J]. 中国食用菌, 2025, 44(04): 31-36. |
| [9] | 陈扬, 王鑫, 普普赤, 等. 一株野生花脸香蘑的鉴定及生物学特性 [J]. 食用菌, 2025, 47(04): 27-34. |
| [10] | 魏洁, 孙叶光, 涂镜, 等. 野生花脸香蘑分离鉴定及其培养基优化 [J]. 食用菌, 2025, 47(05): 33-41. |
| [11] | 何雪梅, 赵辉, 冯慧, 等. 三个花脸香蘑菌株母种的培养条件筛选试验 [J]. 四川农业科技, 2025, (11): 30-36. |
| [12] | 张秀伟, 鲍菊, 张万萍, 等. 贵州4株花脸香蘑的分离鉴定及秋栽驯化 [J]. 中国食用菌, 2022, 41(10): 26-30. |
| [13] | 崔晓, 丛倩倩, 王庆武, 等. 一株野生花脸香蘑的鉴定及其液体培养基的筛选 [J]. 中国食用菌, 2022, 41(08): 17-20+33. |
| [14] | 李菲菲, 郑永标. 花脸香蘑的化学成分及生物活性研究进展 [J]. 食药用菌, 2024, 32(06): 379-385. |
| [15] | 鲁铁, 董梦帆, 王合, 等. 伏牛山地区花脸香蘑液体发酵菌丝体多糖提取工艺优化及活性研究 [J]. 河南城建学院学报, 2025, 34(03): 117-126. |
| [16] | 王艺学, 赵辉, 李彪, 等. 花脸香蘑E70菌株发酵条件优化及其胞外多糖生物活性 [J]. 食品研究与开发, 2024, 45(20): 125-134. |
| [17] | 贺永国, 耿秀英, 姚春, 等. 白参菌菌糠高效栽培花脸香蘑技术 [J]. 食用菌, 2020, 42(06): 53-54+56. |
| [18] | 王丽宁, 黄清铧, 蒋丽群, 等. 花脸香蘑过氧化氢酶基因鉴定及表达分析 [J]. 食用菌学报, 2022, 29(05): 12-21. |
| [19] | 范敏, 吴克刚, 王丽宁, 等. 花脸香蘑荧光定量PCR内参基因筛选及多糖合成代谢相关基因表达量测定 [J]. 食用菌学报, 2021, 28(05): 20-28. |
| [20] | 张秀伟, 牛力立, 蔡甫格. 花脸香蘑交配型研究 [J]. 食用菌学报, 2025, 32(04): 9-12. |
| [21] | 梁敏, 李楠楠, 邹东恢. 蔗糖酶的提取工艺及性质研究 [J]. 湖北农业科学, 2010, 49(09): 2218-2220. |
| [22] | GIBSON S I. Plant sug-ar-response pathways: Part of a complex regulatory web [J]. Plant Physiol, 2000, 124: 1532-1539. |
| [23] | IRAQI D, TREMBLAY F M. Analysis of carbohydrate metabolism enzymes and cellular contents of sugars and pro-teins during spruce somatic embryogenesis suggests a regulato-ry role of exogenous sucrose in embryo development [J]. J Exp Bot, 2001, 52: 2301-2311. |
