大 (古) 松树树干注药残留量检测技术研究
1. 重庆师范大学, 生命科学学院, 重庆 401331
2. 媒介生物控制和利用重庆市重点实验室, 重庆 401331
3. 重庆市森林病虫防治检疫站, 重庆 408099
4. 重庆师范大学, 昆虫分子生物学研究所, 重庆 401331
| 摘 要： | 注干施药技术是一种主动预防松材线虫病的技术。研究选取了忠县、巴南、北碚区缙云山国家级自然保护区、武隆、梁平、合川六个区县（单位）的大（古）马尾松（Pinus massoniana）为研究对象，采用注干施药方法明确了2.3%甲维盐及5%阿维菌素对松材线虫病的预防效果，采用高效液相色谱测定了注干施药180d和360d后马尾松的甲维盐的残留量。结果表明，甲维盐及阿维菌素注干施药对忠县、巴南、北碚区缙云山国家级自然保护区、武隆、梁平、合川六个区县（单位）的大（古）马尾松林的松材线虫病防治效果显著，连续2a预防效果均达到99%以上；此外，建立了马尾松中甲维盐及阿维菌素残留检测方法，侧枝中甲维盐及阿维菌素的添加回收率都超过80%，甲维盐及阿维菌素分析方法满足残留检测要求；注干后180d，甲维盐及阿维菌素在松树侧枝上的残留浓度最高处分别为2.93±1.63mg kg-1、2.93±0.69mg kg-1，第二年时，残留浓度降低，注射甲维盐及阿维菌素的松树侧枝内残留浓度最高处分别为1.93±1.4mg kg-1、0.71±0.49mg kg-1以上。 |
| 关 键 词： | 松材线虫病; 甲维盐; 阿维菌素; 残留动态 |
| DOI： | 10.57237/j.life.2024.02.002 |
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收稿日期: 2024-03-13
接受日期: 2024-04-08
在线出版日期: 2024-04-13
Study on the Detection Technology of Injection Residue in the Trunk of Large (Ancient) Pine
1. College of Life Science, Chongqing Normal University, Chongqing 401331, China
2. Chongqing Station of Forest Pests Control and Quarantine, Chongqing 408099, China
| Abstract: | The injection-dry application technique is a proactive technique to prevent Pine wilt disease. The study selected Zhongxian, Banan, Beibei District Jinyun Mountain National Nature Reserve, Wulong, Liangping, Hechuan six districts and counties (units) of the big (ancient) Pine (Pinus massoniana) as the research object, the use of injection of dry application method to clarify the prevention of Pine wilt disease by 2.3% Emamectin Benzoate and 5% abamectin, the use of high performance liquid chromatography determination of the injection of dry application of 180d and High performance liquid chromatography (HPLC) was used to determine the residue of Emamectin Benzoate in Pinus sylvestris after 180d and 360d of dry application. The results showed that Emamectin Benzoate and abamectin injection dry application of six districts and counties (units) of Zhongxian, Banan, Beibei District Jinyun Mountain National Nature Reserve, Wulong, Liangping, Hechuan, large (ancient) Pine wilt disease prevention and control effect is significant, the prevention effect of the consecutive 2a have reached more than 99%; In addition, the establishment of the pony pine in the Emamectin Benzoate and abamectin residue detection method, the side branch in the Emamectin Benzoate and abamectin In addition, a method was established to detect the residues of Emamectin Benzoate and abamectin in Pinus sylvestris, and the recoveries of Emamectin Benzoate and abamectin in the lateral branches exceeded 80%, and the analytical methods of Emamectin Benzoate and abamectin met the requirements of residue detection; the highest residue concentrations of Emamectin Benzoate and abamectin in the lateral branches of Pinus sylvestris were 2.93±1.63mg kg-1 and 2.93±0.69mg kg-1, respectively, 180d after injection drying. In the second year, the residue concentrations decreased, and the highest concentrations were more than 1.93±1.4 mg kg-1 and 0.71±0.49 mg kg-1 in the lateral branches of pine trees injected with mevinphos and abamectin, respectively. |
| Keywords: | Pine Wilt Disease; Emamectin Benzoate; Abamectin; Residue |
基金
| 1. | 树干注药防控大(古)松树松材线虫病的技术推广与示范 (基金号: 渝林推广 [2021-03]) |
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