基于血管新生探讨丹参防治肝纤维化的作用机制
1. 广西中医药大学, 基础医学院生理教研室, 广西南宁 530222
2. 广西中医药大学, 赛恩斯新医药学院医学系, 广西南宁 530222
| 摘 要： | 目的：运用网络药理学和分子对接探究丹参基于血管新生防治肝纤维化的作用机制。方法：通过TCMSP数据库明晰丹参的活性药理成分以及其作用靶点，利用TTD、GeneCards、CTD等数据库获得肝纤维化、血管新生相关靶点，采用Venn图对丹参活性成分作用靶点和肝纤维化及血管新生相关靶点进行交互处理；使用Cytoscape3.9.0软件构建药物-成分-靶点-疾病网络；利用String数据库构建交互靶点互作网络；利用Metascape平台对交互靶点进行GO（Gene Ontology）富集分析和KEGG（Kyoto Encyclopedia of Genes and Genomes）信号通路分析，明确丹参基于血管新生防治肝纤维化的分子机制。结果：通过TCSMP数据库筛选出丹参活性成分65个，作用潜在靶点132个，通过GeneCards、TTD、CTD数据库分别筛选出肝纤维化和血管新生靶点4841、1200个，映射取交集得到丹参影响血管新生防治肝纤维化的共同靶点50个，构建丹参-成分-靶点-疾病网络图，并对共同靶点蛋白进行PPI网络分析，得到关键靶点。对共同靶点在Metascape平台进行GO和KEGG信号通路富集分析，显示PI3K-Akt信号通路、糖尿病并发症中的AGE-RAGE信号通路、MAPK信号通路、VEGF信号通路等信号通路可能参与丹参调节血管新生防治肝纤维化的不同环节。分子对接结果显示良好。结论：丹参的多种主要活性成分通过多通路发挥抗血管新生的作用，从而防治肝纤维化。 |
| 关 键 词： | 肝纤维化; 血管新生; 丹参; 网络药理学 |
| DOI： | 10.57237/j.mrf.2023.04.006 |
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收稿日期: 2023-11-07
接受日期: 2023-12-06
在线出版日期: 2023-12-27
To Explore the Mechanism of Salvia Miltiorrhiza in Preventing Liver Fibrosis Based on Angiogenesis
1. Department of Physiology, School of Basic Medicine, Guangxi University of Chinese Medicine, Nanning 530222, China
2. Department of Medicine, Faculty of Chinese Medicine Science, Guangxi University of Chinese Medicine, Nanning 530222, China
| Abstract: | Objective: Network pharmacology and molecular docking were used to explore the mechanism of Salvia miltiorrhiza in the prevention and treatment of liver fibrosis based on angiogenesis. Method: The active pharmacological components and their action targets of Salvia miltiorrhiza were clarified by TCMSP database, and the targets related to hepatic fibrosis and angiogenesis were obtained by TTD, GeneCards, CTD and other databases. The active components of Salvia miltiorrhiza and the targets related to liver fibrosis and angiogenesis were interactively processed by Venn map. The drug-component-target-disease network was constructed by Cytoscape3.9.0 software, and the interaction target network was constructed by using String database. The interaction targets were analyzed by GO (Gene Ontology) enrichment analysis and KEGG (Kyoto Encyclopedia of Genesand Genomes) signal pathway analysis using Metascape platform to clarify the molecular mechanism of Salvia miltiorrhiza in the prevention and treatment of liver fibrosis based on angiogenesis. Result: 65 active components and 132potential targets of Salvia miltiorrhiza were screened from TCSMP database, 4841 and 1200 targets of hepatic fibrosis and angiogenesis were screened by GeneCards, TTD and CTD database respectively, 50 common targets of Salvia miltiorrhiza affecting angiogenesis and prevention and treatment of liver fibrosis were obtained by mapping, and the Salvia miltiorrhiza-component-target-disease network map was constructed. The common target proteins were analyzed by PPI network, and the key targets were obtained. The enrichment analysis of GO and KEGG signal pathways on the Metascape platform showed that PI3K-Akt signal pathway, AGE-RAGE signal pathway in diabetic complications, MAPK signal pathway, VEGF signal pathway and other signal pathways may be involved in different aspects of Salvia miltiorrhiza regulating angiogenesis and preventing liver fibrosis. The results of molecular docking are good. Conclusion: A variety of main active components of Salvia miltiorrhiza play the role of anti-angiogenesis through multiple pathways, so as to prevent and treat liver fibrosis. |
| Keywords: | Liver Fibrosis; Angiogenesis; Salvia; Network Pharmacology |
基金
| 1. | 国家自然科学基金项目 (82204755, 81960751) |
| 2. | 广西自然科学基金青年基金项目 (2023GXNSFBA297094) |
| 3. | 广西中医药大学赛恩斯新医药学院国家级大学生创新创业训练项目 (202313643012) |
| 4. | 广西中医药大学国家级大学生创新创业训练项目 (202310600020) |
| 5. | 广西中医药大学青年基金项目 (2022QN008; 2022MS024) |
| 6. | 广西中医药大学赛恩斯新医药学院科研项目 (2022MS002; 2022MS008; 2022QJ001) |
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