基于TCGA数据库构建膀胱尿路上皮癌缺氧相关lncRNA预后模型
1. 中山大学, 肿瘤防治中心肿瘤内科华南肿瘤学国家重点实验室肿瘤医学协同创新中心, 广东广州 510030
2. 中山大学, 附属第三医院泌尿外科, 广东广州 510000
| 摘 要： | 目的：探讨缺氧调节相关lncRNA与膀胱尿路上皮癌患者预后和治疗的关系，为临床医学提供新思路。方法：临床和基因组数据来自癌症基因组图谱（TCGA）数据库，缺氧相关基因来自（GSEA）数据库。预后模型是通过共表达分析和Cox回归分析构建的。通过GO、KEGG和肿瘤突变负荷（TMB）分析揭示缺氧相关lncRNA在肿瘤免疫环境中的作用。结果：构建了包含6个与缺氧相关的lncRNA的预后模型。我们发现低风险患者的总生存期（OS）和无进展生存期（PFS）更高。ROC、独立预测分析、C指数和列线图表明缺氧相关的lncRNA模型可以独立预测患者的预后。TMB分析提示缺氧相关lncRNA的生物学功能与肿瘤免疫有关，低风险并具有高TMB患者预后更佳。结论：我们为膀胱尿路上皮癌患者构建了缺氧相关的lncRNA预后模型，低风险及高TMB患者具有更长的总体生存（OS）。 |
| 关 键 词： | 缺氧; 膀胱尿路上皮癌; 长链非编码RNA; 预后风险模型; 肿瘤突变负荷 |
| DOI： | 10.57237/j.wjcm.2023.03.003 |
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收稿日期: 2023-07-04
接受日期: 2023-08-31
在线出版日期: 2023-09-08
Construction of a Hypoxia-related lncRNA Prognostic Model for Bladder Urothelial Carcinoma Based on TCGA Database
1. Department of Medical Oncology, State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Sun Yat-sen University Cancer Center, Guangzhou 510030, China
2. Department of Urology, The Third Affiliated Hospital, Sun Yat-sen University, Guangzhou 510000, China
| Abstract: | Objective: To investigate the relationship between hypoxia regulation-related lncRNA and the prognosis and treatment of patients with bladder urothelial carcinoma, so as to provide new ideas for clinical medicine. Methods: Clinical and genomic data were obtained from The Cancer Genome Atlas (TCGA) database, and hypoxia-related genes were obtained from the (GSEA) database. Prognostic models were constructed by co-expression analysis and Cox regression analysis. The roles of hypoxia-related lncRNAs in the tumor immune environment were revealed by GO, KEGG and tumor mutational burden (TMB) analysis. Results: A prognostic model containing 6 hypoxia-related lncRNAs was constructed. We found that low-risk patients had better overall survival (OS) and progression-free survival (PFS). ROC, independent predictive analysis, C-index, and nomogram indicated that hypoxia-related lncRNA models could independently predict patient outcomes. TMB analysis suggested that the biological function of hypoxia-related lncRNA was related to tumor immunity, and patients with low risk and high TMB had better prognosis. Conclusion: We constructed a hypoxia-related lncRNA prognostic model for patients with bladder urothelial carcinoma, and patients with low risk and high TMB had longer overall survival (OS). |
| Keywords: | Hypoxia; Urothelial Carcinoma of the Bladder; Long Noncoding RNA; Prognostic Risk Models; Tumor Mutational Burden |
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