自动菌落计数仪在乳制品检测中的应用
1. 蒙牛乳业（沈阳）有限责任公司, 辽宁沈阳 110122
2. 内蒙古蒙牛乳业（集团）股份有限公司, 内蒙古呼和浩特 011517
| 摘 要： | 目的：为提高计数效率，实现原始数据的可追溯性，通过对自动菌落计数仪的适用性、准确性、精密度和重复性进行试验验证，确定其在乳制品和原辅料样品检测中菌落计数的适用情况。方法：选取平板倾注法和测试片法进行验证测试，包括嗜冷菌、需氧芽孢、嗜热需氧芽孢，乳酸菌计数、菌落总数、大肠菌群、霉菌、酵母菌、金黄色葡萄球菌等项目，采用人工计数与自动菌落计数仪计数进行对比，确定其可行性、适用性和准确性。结果：正常的无背景干扰的培养皿菌落计数和3M测试片菌落计数对比验证,目测法与仪器法进行配对t检验，P=0.17,大于0.05，在统计学上结果无显著性差异，有颜色的培养基对自动计数无显著影响。对于针尖状菌落、菌落与培养基颜色接近、菌落轮廓不明显、菌落较大蔓延且菌落边缘不规则、培养基中同时存在食品颗粒与菌落时，或菌落颜色与食品颗粒颜色接近时，仪器计数效果较差不能满足准确性要求。结论：对于质地均匀的样品，自动菌落计数准确度和精密度均比较好，工作效率高节省了实验人员的操作时间，且实验原始数据及图像可在仪器中长期保存，是实验室值得推广的计数方法。但对于试验验证中有干扰的样品和培养基，可采取适当措施改变计数条件，人工修正仪器计数结果，但计数效率会有所降低，实验室可视情况选择使用。 |
| 关 键 词： | 乳制品; 自动菌落计数; 适用性; 效率 |
| DOI： | 10.57237/j.wjfse.2022.01.002 |
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收稿日期: 2022-09-20
接受日期: 2022-10-20
在线出版日期: 2022-11-01
Application of Automatic Colony Counter in the Detection of Dairy Products
1. Mengniu Dairy (Shenyang) Co., LTD., Shenyang 110122, China
2. Inner Mongolia Mengniu Dairy (Group) Co., LTD., Hohhot 011517, China
| Abstract: | Objective: To improve the counting efficiency and realize the traceability of raw data. The applicability, accuracy, precision and repeatability of the automatic colony counter were verified by experiments, Determine the applicability of colony count in the detection of dairy products and raw and auxiliary materials. Methods: The plate pouring method and test piece method are selected for verification test, including psychrophilic bacteria, aerobic spores, thermophilic aerobic spores, lactic acid bacteria count, total number of colonies, coliform, mold, yeast, Staphylococcus aureus and other items. The manual count is compared with the automatic colony counter to determine its feasibility, applicability and accuracy. Results: The colony count of normal Petri dish without background interference was compared with that of 3M test piece. The paired t-test was carried out by visual method and instrument method, P = 0.17, greater than 0.05. There was no significant difference in statistical results, and the colored medium had no significant effect on automatic counting. The counting effect of the instrument is poor when there are needle shaped colonies, the color of colonies is close to that of the culture medium, the contour of colonies is not obvious, the colonies spread greatly and the edges of colonies are irregular, there are food particles and colonies in the culture medium at the same time, or the color of colonies is close to that of food particles, which can not meet the accuracy requirements. Conclusion: For samples with uniform texture, the accuracy and precision of automatic colony counting are relatively good, the work efficiency is high, the operation time of experimental personnel is saved, and the original experimental data and images can be stored in the instrument for a long time. It is a counting method worthy of popularization in the laboratory. However, for the samples and culture medium with interference in the test verification, appropriate measures can be taken to change the counting conditions and manually correct the counting results of the instrument, but the counting efficiency will be reduced, and the laboratory can choose to use it according to the situation. |
| Keywords: | Dairy; Automatic Colony Count; Applicability; Efficiency |
基金
| 1. | 国家重点研发计划食品安全关键技术研发-食品安全监测与控制技术的全链条区域综合示范-食品安全全程监测控制技术保障体系构建方法研究 (2019YFC1606503). |
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