多管发酵法测定食品中大肠菌群的方法学验证及质量控制
赣州美科检测科技有限公司, 江西赣州 341000
| 摘 要： | 提供客观证据以证明试验能够正确运用多管发酵法测定食品中大肠菌群，确保结果的准确性，并对不确定度、注意事项等方面进行探讨，为多管发酵法测定大肠菌群的准确性提供一定参考和技术保障，以期更好服务于食品监测工作。采用多管发酵法测定食品中大肠菌群，参考RB/T 033—2020《微生物检测方法确认与验证指南》，通过使用低、中、高3个接种水平人为污染的样品，验证方法的估计偏差、精密度等，并对不确定度、注意事项和优缺点等方面进行探讨。结果表明，该方法低、中、高3个接种水平与非选择培养基计数结果的估计偏差分别为0.180、0.097、0.064，精密度分别9.8%、1.4%、5.2%。验证参数估计偏差与精密度等均符合要求，多管发酵法可在实验室开展并保证结果的准确性。 |
| 关 键 词： | 方法学验证; 食品; 大肠菌群; 多管发酵法 |
| DOI： | 10.57237/j.wjfse.2023.03.001 |
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收稿日期: 2023-05-31
接受日期: 2023-07-14
在线出版日期: 2023-07-24
Method Validation and Quality Control for Determination of Coliform in Food by Multi-tube Fermentation
Ganzhou Meike Testing Technology Co., Ltd., Ganzhou 341000, China
| Abstract: | Objective evidence is provided to prove that the experiment can correctly use the multi-tube fermentation method to determine coliform in food, to ensure the accuracy of the results, and uncertainty, precautions and other aspects are discussed, to provide a certain reference and technical guarantee for the accuracy of the multi-tube fermentation method to measure coliform, hoping to better serve the food monitoring work. Coliform in food was determined by multi-tube fermentation method, and the estimation deviation and precision of the method were verified by using artificially contaminated samples at low, medium and high inoculation levels according to RB/T 033—2020 Guidelines for Confirmation and Verification of Microbial Detection Methods. The uncertainty, precautions, advantages and disadvantages of the method were discussed. Results showed that the estimated deviations between low, medium and high inoculation levels and the results of non-selective medium count were 0.18, 0.097 and 0.064, and the precision was 9.8%, 1.4% and 5.2%, respectively. The estimation deviation and precision of verification parameters met the requirements. The multi-tube fermentation method can be carried out in laboratory and ensure the accuracy of the results. |
| Keywords: | Methodological Verification; Food; Coliform Bacteria; Multi-tube Fermentation |
参考文献
| [1] | 邓泽, 鲁朝旭, 袁姁, 等. 多管发酵法测定水体中粪大肠菌群的方法验证及质量控制 [J]. 科技风, 2021, 4 (10): 181-182. |
| [2] | 徐俊环, 张华. 食品中大肠菌群的测定及分析 [J]. 食品安全导刊, 2018 (06): 101. DOI: 10.16043/j.cnki.cfs.2018.06.075. |
| [3] | 孟庆红, 吕新, 贾培培, 等. Soleris 检测技术在冰淇淋中大肠菌群检测的应用研究 [J]. 中国奶牛, 2014, 34 (2): 14-17. |
| [4] | 陈灿映. 大肠菌群快速检测培养基的研制及评价 [D]. 郑州: 郑州大学, 2012. |
| [5] | 涂楚国. LTSE法快速检测水质、食品、冷饮、餐具大肠菌群的研究 [J]. 中国公共卫生, 1990 (11): 500-502. |
| [6] | 《中国药典》微生物限度检查法 [M]. 中国标准出版社, 2020. |
| [7] | 国家认证认可监督管理委员会. 微生物检测方法确认与验证指南: RB/T 033—2020 [S]. 北京: 中国标准出版社, 2020。 |
| [8] | 中华人民共和国国家卫生和计划生育委员会, 国家市场监督管理总局. 食品安全国家标准 食品微生物学检验大肠菌群计数: GB 4789.3—2016 [S]. 北京: 中国标准出版社, 2017. |
| [9] | 食品微生物定量检测的测量不确定度评估指南: RB/T 151—2016 [S]. 国家认证认可监督管理委员会, 2016. |
| [10] | 安春艳, 井良义, 陈卓等.药品微生物经典分析方法的验证探讨 [J]. 中国药品标准, 2023, 24 (02): 109-116. DOI: 10.19778/j.chp.2023.02.001. |
| [11] | 侯雪, 郑卫东, 胡莉, 等. 浅析化学检测实验室的方法确认和方法验证 [J]. 实验室研究与探索, 2016, 35 (2): 255-258. |
| [12] | 刘艳霞, 梁文文, 卢仑等. 浅谈食品检测实验室的方法验证和方法确认 [J]. 食品安全导刊, 2023 (12): 162-164. DOI: 10.16043/j.cnki.cfs.2023.12.030. |
| [13] | 张贵刚, 黄博珠. 粪大肠菌群多管发酵法不确定度的评定 [J]. 资源节约与环保, 2018 (8): 37, 48. |
| [14] | 黎尧, 张绍斌. 多管发酵法测定水质粪大肠菌群的探讨 [J]. 环境与发展, 2019 (5): 116-118. |
| [15] | 付伟超, 郝凤霞. 检测实验室方法验证和方法确认探讨 [J].轻工标准与质量, 2018 (04): 59-61. DOI: 10.19541/j.cnki.issn1004-4108.2018.04.020. |
