Life Science and Technology is an international, peer-reviewed open access journal dedicated to advancing research the field of life sciences and technology. The journal provides a rapid publication process to ensure wide dissemination of high-quality articles to scientists, professionals, and interested individuals worldwide. Our goal is to serve as an efficient, reliable, and trusted platform for scholars and readers, publishing cutting-edge research in the field.
Abstract: In this study, according to the issue that the nitrogen supply response characteristics of winter wheat/summer maize across whole year in Hebei plain is needed to be further clarified, the effects of different nitrogen distribution modes on nitrogen uptake, related physiological parameters and yield traits of these crops were investigated. The results showed that compared with control (CK, the ratio of basal application to topdressing 5:5), the population leaf area index (LAI) and dry mass of wheat and maize increased in treatment 1 (T1, the ratio of basal application to topdressing 6:4) at early growth stage (jointing stage). In addition, the photosynthetic parameters (Chl content, Pn and gs), nitrogen content and nitrogen accumulation, nitrate reductase (NR) and glutamine synthase (GS) activities were also increased. However, the above population traits, plant photosynthetic parameters, nitrogen uptake and accumulation, and nitrogen assimilation enzyme activities during the late growth stages of this treatment were worse than those of CK. In contrast to T1, the above traits of wheat and maize in treatment 2 (T2, the ratio of basal application and topdressing 4:6) were worse than CK at early growth stage, but significantly better than CK at the late growth stages (flowering to maturity). According to the yield behavior at maturity stage, the ranking of the treatments was shown to be T2>CK>T1. Gene expression analysis showed that the expression levels of NRT genes TaNRT1.1 and TaNRT2.1 in wheat and NRT genes ZmNRT1.2 and ZmNRT2.1 in maize were involved in nitrogen uptake at flowering stage; those of wheat NR gene TaNR3 and maize NR gene ZmNR3.1 were tightly associated with nitrate reductase (NR) activities; the wheat GS gene TaGS2.2 and the maize GS gene ZmGS2 were related to the activities of glutamine synthetase (GS). These results indicated that the expression levels of these genes had crucial effects on the nitrogen uptake and assimilation characteristics of wheat and maize plants. Our investigation suggested that T2 treatment significantly enhanced the expression levels of specific genes associated with nitrogen uptake and assimilation. It improves the nitrogen utilization and related physiological parameters during the late growth stages by which to enhance the nitrogen efficiency and yield formation ability of the winter wheat/summer maize plants.Abstract: In this study, according to the issue that the nitrogen supply response characteristics of winter wheat/summer maize across whole year in Hebei plain is needed to be further clarified, the effects of different nitrogen distribution modes on nitrogen uptake, related physiological parameters and yield traits of these crops were investigated. The resu...Learn More
Abstract: Objective: To explore the regulatory pathways and the molecular targets of Chaihu Guizhi Ganjiang Decoction (CGGD) against obesity and related metabolic disorders (OMD) by network pharmacology and molecular docking. Methods: The targets and main active components of CGGD and OMD were extracted from TCMSP, SymMap, BATMAN, DisGeNET and GeneCards databases. Disease and compound targets were intersected by Venn diagram to get potential therapeutic targets of CGGD. STRING and Cytoscape software were used to construct target protein interactions and compound-disease-target interactions to identify key CGGD compounds and their regulated pathways in treating OMD, which were further analyzed by GO and KEGG using DAVID (the Database for Annotation, Visualization and Integrated Discovery) and Metascape platforms. Finally, molecular docking was applied to model the interaction of selected molecules with related targets. Results: The CGGD possessed 116 pivotal compounds targeting 52 essential genes involved in OMD. KEGG and GO analysis then revealed that CGGD regulates inflammation, thermogenesis and energy metabolism pathways, whose major targets were docked with CGGD components such as Wogonin, quercetin, Kaempferol and β-Sitosterol by molecular simulation. Conclusion: This study illuminates molecular targets and pathways of CGGD in treating OMD by network pharmacology and multi-pair molecular docking.Abstract: Objective: To explore the regulatory pathways and the molecular targets of Chaihu Guizhi Ganjiang Decoction (CGGD) against obesity and related metabolic disorders (OMD) by network pharmacology and molecular docking. Methods: The targets and main active components of CGGD and OMD were extracted from TCMSP, SymMap, BATMAN, DisGeNET and GeneCards data...Learn More
Abstract: Objective: To analyze the molecular mechanism of lncRNA NEAT1 regulation of liver fibrosis with the help of bioinformatics and experimental validation, hoping to provide new ideas for the prevention and treatment of liver fibrosis and drug development. Methods: Screening lncRNA NEAT1 target miRNAs with the help of Starbase database; screening miRNA target mRNAs using miRDB database; obtaining liver fibrosis-related targets through OMIM, CTD and GeneCards databases; interacting lncRNA NEAT1 regulatory targets with liver fibrosis targets, and performing key target Enrichment analysis was performed. Hepatic stellate cells (HSC) were activated with LPS and divided into blank and model groups. HSC were transfected with siNEAT1 and negative control and divided into siNEAT1 NC+LPS and siNEAT1+LPS. MTT method was used to detect the proliferation of HSC and flow cytometry was used to detect the apoptosis rate of HSC; molecular biology was used to detect α-SMA and Collagen I expression. Results: lncRNA NEAT1 targeted 26 miRNA molecules, miRNA targeted 564 mRNAs, 617 liver fibrosis-related targets, lncRNA NEAT1 regulated 20 key targets of liver fibrosis, lncRNA NEAT1 involvement in liver fibrosis may be closely related to its regulation of cellular senescence. lncRNA NEAT1 expression increased significantly upon HSC activation. The proliferation rate of HSC cells was significantly decreased and apoptosis rate was significantly increased after NEAT1 silencing, and the expression of α-SMA and Collagen I was also significantly decreased. Conclusion: NEAT1 is involved in the development of liver fibrosis by regulating HSC proliferation and apoptosis, and may be closely related to its involvement in the ceRNA network that regulates cellular senescence.Abstract: Objective: To analyze the molecular mechanism of lncRNA NEAT1 regulation of liver fibrosis with the help of bioinformatics and experimental validation, hoping to provide new ideas for the prevention and treatment of liver fibrosis and drug development. Methods: Screening lncRNA NEAT1 target miRNAs with the help of Starbase database; screening miRNA...Learn More
