Life Science and Technology is an international, peer-reviewed open access journal dedicated to advancing research the field of life sciences and technology. The journal provides a rapid publication process to ensure wide dissemination of high-quality articles to scientists, professionals, and interested individuals worldwide. Our goal is to serve as an efficient, reliable, and trusted platform for scholars and readers, publishing cutting-edge research in the field.
Abstract: Objective: To explore the mechanism of Shenqi Dihuang Decoction in treating diabetic nephropathy based on network pharmacology. Methods: The component targets of Shenqi Dihuang Decoction and diabetic nephropathy (DKD) targets were searched and screened by network database. Using Cytoscape3.7.2 software to construct a network of traditional Chinese medicine components-targets; The protein-protein interaction network (PPI) of Shenqi Dihuang Decoction for CRF treatment was constructed using STRING database, and the core targets were found out through topological analysis. Finally, biological pathway and enrichment analysis were carried out on drug-disease intersection targets. Results: A total of 106 active ingredients of Shenqi Dihuang Decoction were collected. 1532 corresponding targets; DKD disease targets 533; There are 48 common targets for drug components and diseases. Topological analysis was performed with a Degree value 2 times (14.3) higher than the average, and 4 key core targets were finally obtained. Biological process and enrichment analysis show that the prescription mainly involves 13 biological processes, 4 KEGG pathways and 3 typical pathways in treating DKD, mainly involving cell response to hormone stimulation, followed by biological processes such as neuroactive ligand-receptor interaction KEGG pathway, blood circulation, cell response to nitrogen compounds, chemical synaptic transmission, etc. Conclusion: Shenqi Dihuang Decoction mainly acts on PTGS2, NR3C1, NOS3, ESR1 and other targets. Its targets are many and its mechanism is complex. It may play a role mainly through the biological process of regulating the response degree of cells to hormone stimulation.Abstract: Objective: To explore the mechanism of Shenqi Dihuang Decoction in treating diabetic nephropathy based on network pharmacology. Methods: The component targets of Shenqi Dihuang Decoction and diabetic nephropathy (DKD) targets were searched and screened by network database. Using Cytoscape3.7.2 software to construct a network of traditional Chinese ...Learn More
Abstract: The protein kinase type of sucrose non-fermenting-1 related protein kinase 2 (SnRK2) plays an important role in regulating plant growth and development and in mediating plant resistance to abiotic stresses given that it involves transduction of distinct internal and abiotic stress signaling. In this study, TaSnRK2.1, a gene of SnRK2 family in wheat, was investigated focusing on its molecular characterization, expression pattern upon drought stress, and the biological function in mediating plant drought tolerance. At nucleic acid level, TaSnRK2.1 is highly homologous to its counterparts of SnRK2 kinase family genes in species of Polish wheat and barley, whose translated protein harbors a conserved catalytic domain of Ser/Thr kinase domain (aa 62-aa 318). Under a 48 h-regime drought treatment, the transcripts of TaSnRK2.1 showed obvious response in roots and leaves, with the highest expression level reached at 24 h after treatment and maintaining a high level to 48 h under drought condition. Meanwhile, the transcripts of TaSnRK2.1 in both roots and leaves induced by drought were recovered following the normal recovery treatment. Gene transgene analysis indicated that compared with wild type control (WT), the tobacco line with sense-overexpression of TaSnRK2.1 (Sen 1) enlarged plant growth phenotypes, increased dry weights, promoted stomata closing, increased contents of cellular osmtic-regulatory substances including proline, soluble protein, and soluble sugar, enhanced photosynthetic capacity and elevated cellular protective enzyme activities under drought treatment. In contrast, the line with antisense-expression of TaSnRK2.1 (Anti 1) reduced plant growth phenotypes, decreased biomass, slowed stomata closing, decreased the contents of cellular osmtic-regulatory substances, photosynthetic capacity and protective enzyme activities compared with WT plants treated by drought stress. Our investigation suggested that TaSnRK2.1 is sensitive in response to drought signaling at transcriptional level that confers enhanced plant drought resistance by regulating physiological processes associated with stomata movement, cellular osmotic-regulatory substance metabolism, photosynthetsis, and protective enzyme activities.Abstract: The protein kinase type of sucrose non-fermenting-1 related protein kinase 2 (SnRK2) plays an important role in regulating plant growth and development and in mediating plant resistance to abiotic stresses given that it involves transduction of distinct internal and abiotic stress signaling. In this study, TaSnRK2.1, a gene of SnRK2 family in wheat...Learn More
Abstract: BBM (baby boom) is an important embryogenesis transcription factor in plants, which is valuable in molecular research and could improve the transformation efficiency in recalcitrant species. However, in time and dose aspect, which stage and how much BBM transcription factor is necessary for stimulating embryogenesis is still largely unknown in Brassicas. GR system and dexamethasone are important to timing and quantitative analysis of transcription factors. In this study, BP and LR reaction were applied to build czn3999-35s: Omega-BBM-GR construct, which was transformed into E. coli DH5 α and Agrobacterium C58 competent cells by electric shock transformation respectively. The correct strain verified by PCR digestion reaction was kept in glycerol and stored at -80°C. Brassica napus 12075 cotyledon petioles were used as recipient, and eventually 22 transgenic plantlets were obtained from agrobacterium mediated bulk-way transformation. We describe Bulk-Way transformation protocol in detail, and provides valuable materials for analyzing dose and time effect of BBM in Brassicas.Abstract: BBM (baby boom) is an important embryogenesis transcription factor in plants, which is valuable in molecular research and could improve the transformation efficiency in recalcitrant species. However, in time and dose aspect, which stage and how much BBM transcription factor is necessary for stimulating embryogenesis is still largely unknown in Bras...Learn More
Abstract: Aconitum vilmorinianum is an important Chinese medicinal herb, which contains diterpenoid alkaloids and has the functions of antirheumatic activity, improvement in human meridians, relief of swelling and pain, activation of the blood circulation, and inhibition of tumor growth. The biosynthesis of diterpenoid alkaloids requires intermediate products obtained through terpenoid skeleton metabolism, in order to synthesize diterpenoid alkaloids. The synthesis of terpenoid skeletons includes the MVA pathway and the MEP pathway. 1-deoxyglucose-5-phosphate synthase (DXS) is the first rate limiting enzyme gene in the MEP pathway and plays an important regulatory role in the synthesis of terpenoids. Objective: To understand the characteristics of the DXS gene in Polygonummultiflorum. Method: By extracting RNA from the leaves of A. vilmorinianumand reverse transcribing it into cDNA, according to the transcriptome data of A. vilmorinianum, primers were designed to clone the full length of the gene, and the sequence of the coding protein was analyzed by bioinformatics. Construct overexpression vectors and inhibitory expression vectors. Results: The results showed that the coding region of the gene is 1512 bp, with 503 amino acids, molecular formula C2408H3822N652O713S22, molecular weight 54020.10, the isoelectric point pI 6.29, the instability index 35.70, and belongs to the stable protein category. Successfully constructing overexpression vectors and inhibitory expression vectors can provide scientific basis for further research on the function of this gene in the diterpene biosynthesis pathway.Abstract: Aconitum vilmorinianum is an important Chinese medicinal herb, which contains diterpenoid alkaloids and has the functions of antirheumatic activity, improvement in human meridians, relief of swelling and pain, activation of the blood circulation, and inhibition of tumor growth. The biosynthesis of diterpenoid alkaloids requires intermediate product...Learn More
